"JkDefrag is a disk defragmenter and optimizer for Windows 2000/2003/XP/Vista/2008/X64. Completely automatic and very easy to use, fast, low overhead, with several optimization strategies, and can handle floppies, USB disks, memory sticks, and anything else that looks like a disk to Windows. Included are a Windows version, a commandline version (for scheduling by the task scheduler or for use from administrator scripts), a screensaver version, a DLL library (for use from programming languages), versions for Windows X64, and the complete sources."
Homepage: http://www.kessels.com/JkDefrag/
JkDefrag Gui: http://www.emro.nl/freeware/ (Combine jkdefrag with other tools)